|
KMID : 0881720200350030352
|
|
Journal of Food Hygiene and Safety
2020 Volume.35 No. 3 p.252 ~ p.260
|
|
|
Determination of Vitamin B 12 and Biotin in Foods for Special Dietary Uses with Immunoaffinity Column
|
|
|
|
|
Abstract
|
|
|
|
This study was conducted to improve the standard method for vitamin B 12 and biotin contained in foods for special dietary uses to ensure the specificity of the complex matrix properties of foods. For the food code, the test method was improved to determine vitamin B 12 and biotin by high-performance liquid chromatography (HPLC)-UV using columnswitching after concentration using immunoaffinity column. The immunoaffinity columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest so that it can be used to concentrate the vitamin B 12 and biotin and remove interferences from the food extracts. Moreover, validation of advanced new methods was carried out to support the suitability of the proposed analytical procedure (specificity, linearity, detection limits (LOD), quantitative limits (LOQ), accuracy, and precision). The improved analytical method is being used to monitor relevant food items on sale. The results of this study showed that the new analytical method is suitable and appropriate for managing food intended for special dietary uses.
|
|
|
KEYWORD
|
|
|
: Vitamin B12, Biotin, Column-switching HPLC, Foods for special dietary uses, Immunoaffinity column
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|